The role of a polymeric nanoparticle gene delivery method in bone development

Is there a regenerative medicine “magic bullet”? A cutting-edge gene therapy for repairing tissues damaged by disease or trauma has long been a goal of scientists. Now that a research team has created a polymeric gene delivery therapy that encourages new bone formation following traumatic inflammation, that wish may come true.

Researchers from Tokyo Medical and Dental University (TMDU) discovered in a study that was published in the International Journal of Molecular Sciences that a gene delivery therapy can effectively reduce inflammation and speed up tissue healing following tooth extraction.

Tooth extraction is a common procedure in dental medicine.Sores form in the socket after a tooth is extracted, which initiates a physiological healing process in which damaged soft and hard tissues are rebuilt. Hemostasis, or stopping the bleeding from blood clots, initiates an inflammatory phase in one phase of wound healing.

“This is the first in-vivo investigation to demonstrate the efficacy of NF-B decoy ODNs delivered via a copolymer vector to improve bone repair following tooth extraction,”

Lead author Takashi Ono.

Although prolonged use may result in complications, this may assist in stopping further bleeding. Too much inflammation after a tooth extraction can lead to residual ridge resorption, or the loss of bone in the jaw, which can have a negative and aggravating effect on dental surgery.

A signaling pathway involving the protein complex NF-B (nuclear factor kappa B) is responsible for the ongoing process of bone remodeling. It regulates the expression of genes that control bone healing and inflammation. “The use of an NF-B decoy oligodeoxynucleotide (ODN) to treat bone resorption is well-known; however, little research has been done on the use of an NF-B decoy oligodeoxynucleotide (ODN),” says lead author Takashi Ono.

Molar extraction surgery was used to remove teeth in this study. A quality/drug conveyance framework was created using a productive copolymer vector — a kind of poly(lactic-co-glycolic corrosive)—to convey NF-B fake ODNs (twofold abandoned DNA sections) to the extraction attachment of rodents, and their helpful impacts were explored. Using a copolymer vector, it was discovered that local administration of NF-B decoy ODNs effectively promoted bone formation at the extraction socket and inhibited bone resorption.

Gene delivery therapy also kept inflammation under control.  According to Ono, “This is the first in-vivo study to demonstrate that NF-B decoy ODNs administered by a copolymer vector are effective in promoting bone healing after tooth extraction.”

Innovative gene therapy to stop residual ridge resorption following surgical tooth extraction may result from this study’s findings.

More information: Albert chun-shuo Huang et al, NF-κB Decoy ODN-Loaded Poly(Lactic-co-glycolic Acid) Nanospheres Inhibit Alveolar Ridge Resorption, International Journal of Molecular Sciences (2023). DOI: 10.3390/ijms24043699

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